• Western blot analysis of various lysates using POU5F1B Rabbit polyclonal antibody (STJ118151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-POU5F1B antibody (1-140) (STJ118151)

SKU:
STJ118151

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU5F1B (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU5F1B
Gene ID: 5462
Uniprot ID: P5F1B_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POU5F1B (NP_001153014.1).
Immunogen Sequence: MAGHLASDFAFSPPPGGGGD GPWGAEPGWVDPLTWLSFQG PPGGPGIGPGVGPGSEVWGI PPCPPPYELCGGMAYCGPQV GVGLVPQGGLETSQPESEAG VGVESNSNGASPEPCTVPPG AVKLEKEKLEQNPEKSQDIK
Tissue Specificity Detected at the mRNA level in several cancer tissues (breast, uterine cervix, lung, thyroid gland, esophagus, colon, urinary bladder, and glioma), but absent in normal tissues.
Function Shows weak transcriptional activator activity.
Protein Name Putative Pou Domain - Class 5 - Transcription Factor 1b
Oct4-Pg1
Octamer-Binding Protein 3-Like
Octamer-Binding Transcription Factor 3-Like
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Putative Pou Domain - Class 5 - Transcription Factor 1b antibody
Anti-Oct4-Pg1 antibody
Anti-Octamer-Binding Protein 3-Like antibody
Anti-Octamer-Binding Transcription Factor 3-Like antibody
Anti-POU5F1B antibody
Anti-OCT4PG1 antibody
Anti-OTF3C antibody
Anti-OTF3P1 antibody
Anti-POU5F1P1 antibody
Anti-POU5FLC20 antibody
Anti-POU5FLC8 antibody

Information sourced from Uniprot.org

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