• Western blot analysis of various lysates using PNN Rabbit polyclonal antibody (STJ119244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PNN antibody (1-100) (STJ119244)

SKU:
STJ119244

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNN (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNN
Gene ID: 5411
Uniprot ID: PININ_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PNN (NP_002678.2).
Immunogen Sequence: MAVAVRTLQEQLEKAKESLK NVDENIRKLTGRDPNDVRPI QARLLALSGPGGGRGRGSLL LRRGFSDSGGGPPAKQRDLE GAVSRLGGERRTRRESRQES
Tissue Specificity Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.
Function Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene.the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
Protein Name Pinin
140 Kda Nuclear And Cell Adhesion-Related Phosphoprotein
Desmosome-Associated Protein
Domain-Rich Serine Protein
Drs Protein
Drsp
Melanoma Metastasis Clone A Protein
Nuclear Protein Sdk3
Sr-Like Protein
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Cell Junction
Desmosome
Cell-Cell Contact Area
Predominantly Desmosome Of Intercellular Adherens Junction
Not A Nucleocytoplasmic Shuttling Protein
Alternative Antibody Names Anti-Pinin antibody
Anti-140 Kda Nuclear And Cell Adhesion-Related Phosphoprotein antibody
Anti-Desmosome-Associated Protein antibody
Anti-Domain-Rich Serine Protein antibody
Anti-Drs Protein antibody
Anti-Drsp antibody
Anti-Melanoma Metastasis Clone A Protein antibody
Anti-Nuclear Protein Sdk3 antibody
Anti-Sr-Like Protein antibody
Anti-PNN antibody
Anti-DRS antibody
Anti-MEMA antibody

Information sourced from Uniprot.org

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