• Western blot analysis of extracts of various cell lines, using PICALM antibody (STJ112590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PICALM antibody (290-460) (STJ112590)

SKU:
STJ112590

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PICALM (290-460) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PICALM
Gene ID: 8301
Uniprot ID: PICAL_HUMAN
Immunogen Region: 290-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1).
Immunogen Sequence: IKDSTAASRATTLSNAVSSL ASTGLSLTKVDEREKQAALE EEQARLKALKEQRLKELAKK PHTSLTTAASPVSTSAGGIM TAPAIDIFSTPSSSNSTSKL PNDLLDLQQPTFHPSVHPMS TASQVASTWGGFTPSPVAQP HPSAGLNVDFESVFGNKSTN VIVDSGGFDEL
Tissue Specificity Expressed in all tissues examined.
Function Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
Protein Name Phosphatidylinositol-Binding Clathrin Assembly Protein
Clathrin Assembly Lymphoid Myeloid Leukemia Protein
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9696264
Cellular Localisation Cell Membrane
Membrane
Clathrin-Coated Pit
Golgi Apparatus
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Nucleus
Colocalized With Clathrin In The Golgi Area
Interaction With Pimreg May Target Picalm To The Nucleus In Some Cells
Alternative Antibody Names Anti-Phosphatidylinositol-Binding Clathrin Assembly Protein antibody
Anti-Clathrin Assembly Lymphoid Myeloid Leukemia Protein antibody
Anti-PICALM antibody
Anti-CALM antibody

Information sourced from Uniprot.org