Host: | Rabbit |
Applications: | WB |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-PICALM (290-460) is suitable for use in Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:1000-1:2000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | PICALM |
Gene ID: | 8301 |
Uniprot ID: | PICAL_HUMAN |
Immunogen Region: | 290-460 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1). |
Immunogen Sequence: | IKDSTAASRATTLSNAVSSL ASTGLSLTKVDEREKQAALE EEQARLKALKEQRLKELAKK PHTSLTTAASPVSTSAGGIM TAPAIDIFSTPSSSNSTSKL PNDLLDLQQPTFHPSVHPMS TASQVASTWGGFTPSPVAQP HPSAGLNVDFESVFGNKSTN VIVDSGGFDEL |
Tissue Specificity | Expressed in all tissues examined. |
Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). |
Protein Name | Phosphatidylinositol-Binding Clathrin Assembly Protein Clathrin Assembly Lymphoid Myeloid Leukemia Protein |
Database Links | Reactome: R-HSA-432722 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-9696264 |
Cellular Localisation | Cell Membrane Membrane Clathrin-Coated Pit Golgi Apparatus Cytoplasmic Vesicle Clathrin-Coated Vesicle Nucleus Colocalized With Clathrin In The Golgi Area Interaction With Pimreg May Target Picalm To The Nucleus In Some Cells |
Alternative Antibody Names | Anti-Phosphatidylinositol-Binding Clathrin Assembly Protein antibody Anti-Clathrin Assembly Lymphoid Myeloid Leukemia Protein antibody Anti-PICALM antibody Anti-CALM antibody |
Information sourced from Uniprot.org