| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PICALM (290-460) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PICALM |
| Gene ID: |
8301 |
| Uniprot ID: |
PICAL_HUMAN |
| Immunogen Region: |
290-460 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1). |
| Immunogen Sequence: |
IKDSTAASRATTLSNAVSSL ASTGLSLTKVDEREKQAALE EEQARLKALKEQRLKELAKK PHTSLTTAASPVSTSAGGIM TAPAIDIFSTPSSSNSTSKL PNDLLDLQQPTFHPSVHPMS TASQVASTWGGFTPSPVAQP HPSAGLNVDFESVFGNKSTN VIVDSGGFDEL |
| Tissue Specificity | Expressed in all tissues examined. |
| Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). |
| Protein Name | Phosphatidylinositol-Binding Clathrin Assembly ProteinClathrin Assembly Lymphoid Myeloid Leukemia Protein |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9696264 |
| Cellular Localisation | Cell MembraneMembraneClathrin-Coated PitGolgi ApparatusCytoplasmic VesicleClathrin-Coated VesicleNucleusColocalized With Clathrin In The Golgi AreaInteraction With Pimreg May Target Picalm To The Nucleus In Some Cells |
| Alternative Antibody Names | Anti-Phosphatidylinositol-Binding Clathrin Assembly Protein antibodyAnti-Clathrin Assembly Lymphoid Myeloid Leukemia Protein antibodyAnti-PICALM antibodyAnti-CALM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
