• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Kv4.2/KCND2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-KCND2 antibody (581-630 aa) (STJ93879)

SKU:
STJ93879

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Potassium voltage-gated channel subfamily D member 2 (581-630 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCND2
Gene ID: 3751
Uniprot ID: KCND2_HUMAN
Immunogen Region: 581-630 aa
Specificity: Kv4.2 Polyclonal Antibody detects endogenous levels of Kv4.2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Kv4.2/KCND2 at the amino acid range 581-630
Post Translational Modifications Phosphorylation at Ser-438 in response to MAPK activation is increased in stimulated dendrites. Interaction with KCNIP2 and DPP6 propomtes phosphorylation by PKA at Ser-552. Phosphorylation at Ser-552 has no effect on interaction with KCNIP3, but is required for the regulation of channel activity by KCNIP3. Phosphorylation at Ser-552 leads to KCND2 internalization. Phosphorylated by MAPK in response to signaling via the metabotropic glutamate receptor GRM5. Phosphorylation at Ser-616 is required for the down-regulation of neuronal A-type currents in response to signaling via GRM5.
Function Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. Mediates the major part of the dendritic A-type current I(SA) in brain neurons. This current is activated at membrane potentials that are below the threshold for action potentials. It regulates neuronal excitability, prolongs the latency before the first spike in a series of action potentials, regulates the frequency of repetitive action potential firing, shortens the duration of action potentials and regulates the back-propagation of action potentials from the neuronal cell body to the dendrites. Contributes to the regulation of the circadian rhythm of action potential firing in suprachiasmatic nucleus neurons, which regulates the circadian rhythm of locomotor activity. Functions downstream of the metabotropic glutamate receptor GRM5 and plays a role in neuronal excitability and in nociception mediated by activation of GRM5. Mediates the transient outward current I(to) in rodent heart left ventricle apex cells, but not in human heart, where this current is mediated by another family member. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCND2 and KCND3.channel properties depend on the type of pore-forming alpha subunits that are part of the channel. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes. Interaction with specific isoforms of the regulatory subunits KCNIP1, KCNIP2, KCNIP3 or KCNIP4 strongly increases expression at the cell surface and thereby increases channel activity.it modulates the kinetics of channel activation and inactivation, shifts the threshold for channel activation to more negative voltage values, shifts the threshold for inactivation to less negative voltages and accelerates recovery after inactivation. Likewise, interaction with DPP6 or DPP10 promotes expression at the cell membrane and regulates both channel characteristics and activity.
Protein Name Potassium Voltage-Gated Channel Subfamily D Member 2
Voltage-Gated Potassium Channel Subunit Kv4.2
Database Links Reactome: R-HSA-1296072
Reactome: R-HSA-5576894
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Dendrite
Synapse
Perikaryon
Postsynaptic Cell Membrane
Dendritic Spine
Cell Junction
In Neurons
Primarily Detected On Dendrites
Dendritic Spines And On The Neuron Cell Body
But Not On Axons
Localized Preferentially At The Dendrites Of Pyramidal Cells In The Hippocampus Ca1 Layer
Detected At Gabaergic Synapses
Detected At Cell Junctions That Are Distinct From Synaptic Cell Contacts
Detected In Lipid Rafts
Detected Primarily At The Endoplasmic Reticulum Or Golgi When Expressed By Itself
Interaction With Kcnip1
Kcnip2
Kcnip3 Or Kcnip4 Promotes Expression At The Cell Membrane
Interaction With Dpp6 Or Dpp10 Promotes Expression At The Cell Membrane
Internalized From The Cell Membrane By Clathrin-Dependent Endocytosis In Response To Activation Of Ampa-Selective Glutamate Receptors And Pka-Mediated Phosphorylation At Ser-552
Redistributed From Dendritic Spines To The Main Dendritic Shaft In Response To Activation Of Ampa-Selective Glutamate Receptors And Activation Of Pka
Alternative Antibody Names Anti-Potassium Voltage-Gated Channel Subfamily D Member 2 antibody
Anti-Voltage-Gated Potassium Channel Subunit Kv4.2 antibody
Anti-KCND2 antibody
Anti-KIAA1044 antibody

Information sourced from Uniprot.org

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