| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Peflin (50-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4110RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PEF1 |
| Gene ID: |
553115 |
| Uniprot ID: |
PEF1_HUMAN |
| Immunogen Region: |
50-150 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PEF1 (Q9UBV8). |
| Immunogen Sequence: |
APGGPYGPPAGGGPYGHPNP GMFPSGTPGGPYGGAAPGGP YGQPPPSSYGAQQPGLYGQG GAPPNVDPEAYSWFQSVDSD HSGYISMKELKQALVNCNWS S |
| Post Translational Modifications | Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex. |
| Function | Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport.possibly by inhibiting interaction between PDCD6 and SEC31. |
| Protein Name | PeflinPef Protein With A Long N-Terminal Hydrophobic DomainPenta-Ef Hand Domain-Containing Protein 1 |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumMembranePeripheral Membrane ProteinCytoplasmic VesicleCopii-Coated Vesicle MembraneMembrane-Associated In The Presence Of Ca(2+)Localizes To Endoplasmic Reticulum Exit Site (Eres) |
| Alternative Antibody Names | Anti-Peflin antibodyAnti-Pef Protein With A Long N-Terminal Hydrophobic Domain antibodyAnti-Penta-Ef Hand Domain-Containing Protein 1 antibodyAnti-PEF1 antibodyAnti-ABP32 antibodyAnti-UNQ1845 antibodyAnti-PRO3573 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
