• Western blot analysis of extracts of various cell lines, using PEDF/PEDF/SERPINF1 rabbit polyclonal antibody (STJ11102809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HepG2 cells using PEDF/SERPINF1 rabbit polyclonal antibody (STJ11102809) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-PEDF/SERPINF1 antibody (141-240) (STJ11102809)

SKU:
STJ11102809

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PEDF/SERPINF1 (141-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINF1
Gene ID: 5176
Uniprot ID: PEDF_HUMAN
Immunogen Region: 141-240
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 141-240 of human PEDF/SERPINF1 (NP_002606.3).
Immunogen Sequence: RIVFEKKLRIKSSFVAPLEK SYGTRPRVLTGNPRLDLQEI NNWVQAQMKGKLARSTKEIP DEISILLLGVAHFKGQWVTK FDSRKTSLEDFYLDEERTVR
Tissue Specificity Retinal pigment epithelial cells and blood plasma.
Post Translational Modifications The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Function Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Protein Name Pigment Epithelium-Derived Factor
Pedf
Cell Proliferation-Inducing Gene 35 Protein
Epc-1
Serpin F1
Cellular Localisation Secreted
Melanosome
Enriched In Stage I Melanosomes
Alternative Antibody Names Anti-Pigment Epithelium-Derived Factor antibody
Anti-Pedf antibody
Anti-Cell Proliferation-Inducing Gene 35 Protein antibody
Anti-Epc-1 antibody
Anti-Serpin F1 antibody
Anti-SERPINF1 antibody
Anti-PEDF antibody
Anti-PIG35 antibody

Information sourced from Uniprot.org

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