| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDHX |
| Gene ID: | 8050 |
| Uniprot ID: | ODPX_HUMAN |
| Immunogen Region: | 1-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHX (NP_003468.2). |
| Immunogen Sequence: | MAASWRLGCDPRLLRYLVGF PGRRSVGLVKGALGWSVSRG ANWRWFHSTQWLRGDPIKIL MPSLSPTMEEGNIVKWLKKE GEAVSAGDALCEIETDKAVV TLDASDDGILAKIVVEEGSK NIRLGSLIGLIVEEGEDWKH VEIPKDVGPPPPVSKPSEPR PSPEPQISIPVKKEHIPGTL RFRLSPAARNILEKHSLDAS QGTATGPRGIFTKEDALKLV QLKQTGKITESRPTPAPTA |
| Post Translational Modifications | Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity. |
| Function | Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. |
| Protein Name | Pyruvate Dehydrogenase Protein X Component - MitochondrialDihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase ComplexE3-Binding ProteinE3bpLipoyl-Containing Pyruvate Dehydrogenase Complex Component XProx |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase Protein X Component - Mitochondrial antibodyAnti-Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex antibodyAnti-E3-Binding Protein antibodyAnti-E3bp antibodyAnti-Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X antibodyAnti-Prox antibodyAnti-PDHX antibodyAnti-PDX1 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/93441/374525/STJ114319_1__43866.1713141042.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
