| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Optineurin (Internal Region) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A dilution of 1:2000 is recommended for WB and 1:1000 IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | Optn |
| Gene ID: | 71648 |
| Uniprot ID: | OPTN_MOUSE |
| Immunogen Region: | Internal Region |
| Specificity: | Specific for Optineurin. |
| Immunogen: | A synthetic peptide from internal region of mouse Optineurin conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection. |
| Function | Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and huntingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TFRC/TfR).regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family.targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. |
| Protein Name | Optineurin |
| Database Links | Reactome: R-MMU-2565942 Reactome: -MMU-5205685 Reactome: -MMU-5357905 Reactome: -MMU-5357956 Reactome: -MMU-8854214 Reactome: -MMU-936964 Reactome: -MMU-9824878 |
| Cellular Localisation | Cytoplasm Perinuclear Region Golgi Apparatus Trans-Golgi Network Cytoplasmic Vesicle Autophagosome Recycling Endosome Found In The Perinuclear Region And Associates With The Golgi Apparatus Colocalizes With Myo6 And Rab8 At The Golgi Complex And In Vesicular Structures Close To The Plasma Membrane Localizes To Lc3-Positive Cytoplasmic Vesicles Upon Induction Of Autophagy |
| Alternative Antibody Names | Anti-Optineurin antibody Anti-Optn antibody |
Information sourced from Uniprot.org