• Western blot analysis of extracts of various cell lines, using OPHN1 Antibody (STJ29020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-OPHN1 antibody (1-270) (STJ29020)

SKU:
STJ29020

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OPHN1 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OPHN1
Gene ID: 4983
Uniprot ID: OPHN1_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human OPHN1 (NP_002538.1).
Immunogen Sequence: MGHPPLEFSDCYLDSPDFRE RLKCYEQELERTNKFIKDVI KDGNALISAMRNYSSAVQKF SQTLQSFQFDFIGDTLTDDE INIAESFKEFAELLNEVENE RMMMVHNASDLLIKPLENFR KEQIGFTKERKKKFEKDGER FYSLLDRHLHLSSKKKESQL QEADLQVDKERHNFFESSLD YVYQIQEVQESKKFNIVEPV LAFLHSLFISNSLTVELTQD FLPYKQQLQLSLQNTRNHF
Tissue Specificity Expressed in brain.
Function Stimulates GTP hydrolysis of members of the Rho family. Its action on RHOA activity and signaling is implicated in growth and stabilization of dendritic spines, and therefore in synaptic function. Critical for the stabilization of AMPA receptors at postsynaptic sites. Critical for the regulation of synaptic vesicle endocytosis at presynaptic terminals. Required for the localization of NR1D1 to dendrites, can suppress its repressor activity and protect it from proteasomal degradation.
Protein Name Oligophrenin-1
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Cellular Localisation Postsynapse
Presynapse
Cell Projection
Axon
Dendritic Spine
Dendrite
Cytoplasm
Alternative Antibody Names Anti-Oligophrenin-1 antibody
Anti-OPHN1 antibody

Information sourced from Uniprot.org

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