| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NSMCE1 (7-266) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NSMCE1 |
| Gene ID: |
197370 |
| Uniprot ID: |
NSE1_HUMAN |
| Immunogen Region: |
7-266 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2). |
| Immunogen Sequence: |
RMGVMTDVHRRFLQLLMTHG VLEEWDVKRLQTHCYKVHDR NATVDKLEDFINNINSVLES LYIEIKRGVTEDDGRPIYAL VNLATTSISKMATDFAENEL DLFRKALELIIDSETGFASS TNILNLVDQLKGKKMRKKEA EQVLQKFVQNKWLIEKEGEF TLHGRAILEMEQYIRETYPD AVKICNICHSLLIQGQSCET CGIRMHLPCVAKYFQSNAEP RCPHCNDYWPHEIPKVFDP |
| Post Translational Modifications | Ubiquitinated. |
| Function | RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair. NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair. MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. |
| Protein Name | Non-Structural Maintenance Of Chromosomes Element 1 HomologNon-Smc Element 1 Homolog |
| Database Links | Reactome: R-HSA-3108214 |
| Cellular Localisation | NucleusChromosomeTelomere |
| Alternative Antibody Names | Anti-Non-Structural Maintenance Of Chromosomes Element 1 Homolog antibodyAnti-Non-Smc Element 1 Homolog antibodyAnti-NSMCE1 antibodyAnti-HSPC333 antibodyAnti-HSPC337 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
