Host: | Rabbit |
Applications: | WB |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-iNOS (1-170) is suitable for use in Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:1000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | NOS2 |
Gene ID: | 4843 |
Uniprot ID: | NOS2_HUMAN |
Immunogen Region: | 1-170 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human iNOS (NP_000616.3). |
Immunogen Sequence: | MACPWKFLFKTKFHQYAMNG EKDINNNVEKAPCATSSPVT QDDLQYHNLSKQQNESPQPL VETGKKSPESLVKLDATPLS SPRHVRIKNWGSGMTFQDTL HHKAKGILTCRSKSCLGSIM TPKSLTRGPRDKPTPPDELL PQAIEFVNQYYGSFKEAKIE EHLARVEAVT |
Tissue Specificity | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes. |
Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
Protein Name | Nitric Oxide Synthase - Inducible Hepatocyte Nos Hep-Nos Inducible No Synthase Inducible Nos Inos Nos Type Ii Peptidyl-Cysteine S-Nitrosylase Nos2 |
Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-6785807 Reactome: R-HSA-9033241 Reactome: R-HSA-9636249 |
Cellular Localisation | Cytoplasm Cytosol Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4 Exhibits A More Diffuse Cytosolic Localization |
Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibody Anti-Hepatocyte Nos antibody Anti-Hep-Nos antibody Anti-Inducible No Synthase antibody Anti-Inducible Nos antibody Anti-Inos antibody Anti-Nos Type Ii antibody Anti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibody Anti-NOS2 antibody Anti-NOS2A antibody |
Information sourced from Uniprot.org