• Western blot analysis of extracts of various cell lines, using PAFAH1B1 antibody (STJ114517) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-PAFAH1B1 antibody (1-170) (STJ114517)

SKU:
STJ114517

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAFAH1B1 (1-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAFAH1B1
Gene ID: 5048
Uniprot ID: LIS1_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PAFAH1B1 (NP_000421.1).
Immunogen Sequence: MVLSQRQRDELNRAIADYLR SNGYEEAYSVFKKEAELDVN EELDKKYAGLLEKKWTSVIR LQKKVMELESKLNEAKEEFT SGGPLGQKRDPKEWIPRPPE KYALSGHRSPVTRVIFHPVF SVMVSASEDATIKVWDYETG DFERTLKGHTDSVQDISFDH SGKLLASCSA
Tissue Specificity Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
Function Regulatory subunit (beta subunit) of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)), an enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and participates in PAF inactivation. Regulates the PAF-AH (I) activity in a catalytic dimer composition-dependent manner. Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos. May modulate the Reelin pathway through interaction of the PAF-AH (I) catalytic dimer with VLDLR.
Protein Name Platelet-Activating Factor Acetylhydrolase Ib Subunit Beta
Lissencephaly-1 Protein
Lis-1
Paf Acetylhydrolase 45 Kda Subunit
Paf-Ah 45 Kda Subunit
Paf-Ah Alpha
Pafah Alpha
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5663220
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8854518
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Nucleus Membrane
Redistributes To Axons During Neuronal Development
Also Localizes To The Microtubules Of The Manchette In Elongating Spermatids And To The Meiotic Spindle In Spermatocytes
Localizes To The Plus End Of Microtubules And To The Centrosome
May Localize To The Nuclear Membrane
Alternative Antibody Names Anti-Platelet-Activating Factor Acetylhydrolase Ib Subunit Beta antibody
Anti-Lissencephaly-1 Protein antibody
Anti-Lis-1 antibody
Anti-Paf Acetylhydrolase 45 Kda Subunit antibody
Anti-Paf-Ah 45 Kda Subunit antibody
Anti-Paf-Ah Alpha antibody
Anti-Pafah Alpha antibody
Anti-PAFAH1B1 antibody
Anti-LIS1 antibody
Anti-MDCR antibody
Anti-MDS antibody
Anti-PAFAHA antibody

Information sourced from Uniprot.org

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