• Western blot analysis of various lysates, using NG2/CSPG4 antibody (STJ11104877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NG2/CSPG4 antibody (STJ11104877)

SKU:
STJ11104877

€140.63 - €653.13
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NG2/CSPG4 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cspg4
Gene ID: 121021
Uniprot ID: CSPG4_MOUSE
Immunogen: Recombinant protein of human NG2/CSPG4.
Tissue Specificity Expressed in microcascular pericytes and not endothelial cells.
Post Translational Modifications O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Function Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Protein Name Chondroitin Sulfate Proteoglycan 4
Chondroitin Sulfate Proteoglycan Ng2
Proteoglycan An2
Database Links Reactome: R-MMU-1971475
Reactome: -MMU-2022870
Reactome: -MMU-2022923
Reactome: -MMU-2024101
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Extracellular Side
Apical Cell Membrane
Cell Projection
Lamellipodium Membrane
Cell Surface
Localized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By Prkca
Localizes To The Retraction Fibers
A Fraction May Undergo Cell Surface Proteolysis And Secretion
Localizes To The Plasma Membrane Of Oligodendrocytes
Alternative Antibody Names Anti-Chondroitin Sulfate Proteoglycan 4 antibody
Anti-Chondroitin Sulfate Proteoglycan Ng2 antibody
Anti-Proteoglycan An2 antibody
Anti-Cspg4 antibody
Anti-An2 antibody
Anti-Kiaa4232 antibody
Anti-Ng2 antibody

Information sourced from Uniprot.org

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