| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDE1 |
| Gene ID: | 54820 |
| Uniprot ID: | NDE1_HUMAN |
| Immunogen Region: | 1-110 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1). |
| Immunogen Sequence: | MEDSGKTFSSEEEEANYWKD LAMTYKQRAENTQEELREFQ EGSREYEAELETQLQQIETR NRDLLSENNRLRMELETIKE KFEVQHSEGYRQISALEDDL AQTKAIKDQL |
| Tissue Specificity | Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum. |
| Post Translational Modifications | Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition. |
| Function | Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex. |
| Protein Name | Nuclear Distribution Protein Nude Homolog 1 Nude |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5620912 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-8854518 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Chromosome Centromere Kinetochore Spindle Cleavage Furrow Localizes To The Interphase And S Phase Centrosome During Mitosis Partially Associated With The Mitotic Spindle Concentrates At The Plus Ends Of Microtubules Coincident With Kinetochores In Metaphase And Anaphase In A Cenpf-Dependent Manner Also Localizes To The Cleavage Furrow During Cytokinesis Manner |
| Alternative Antibody Names | Anti-Nuclear Distribution Protein Nude Homolog 1 antibody Anti-Nude antibody Anti-NDE1 antibody Anti-NUDE antibody |
Information sourced from Uniprot.org