| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MPP5 (376-576) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PALS1 |
| Gene ID: |
64398 |
| Uniprot ID: |
PALS1_HUMAN |
| Immunogen Region: |
376-576 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 376-576 of human MPP5 (NP_071919.2). |
| Immunogen Sequence: |
GDILHVISQEDPNWWQAYRE GDEDNQPLAGLVPGKSFQQQ REAMKQTIEEDKEPEKSGKL WCAKKNKKKRKKVLYNANKN DDYDNEEILTYEEMSLYHQP ANRKRPIILIGPQNCGQNEL RQRLMNKEKDRFASAVPHTT RSRRDQEVAGRDYHFVSRQA FEADIAAGKFIEHGEFEKNL YGTSIDSVRQVINSGKICLL S |
| Tissue Specificity | Expressed at the outer limiting membrane in the retina (at protein level). Expressed in T lymphocytes (at protein level). Expressed in the kidney (at protein level). |
| Function | Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface. Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity. Required during embryonic and postnatal retinal development. Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation, cerebellar layer organization and cortical development. Plays a role in neuronal progenitor cell survival, potentially via promotion of mTOR signaling. Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Plays a role in the T-cell receptor-mediated activation of NF-kappa-B. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. Required for the normal polarized localization of the vesicular marker STX4. Required for the correct trafficking of the myelin proteins PMP22 and MAG. Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7. (Microbial infection) Acts as an interaction partner for human coronaviruses SARS-CoV and, probably, SARS-CoV-2 envelope protein E which results in delayed formation of tight junctions and disregulation of cell polarity. |
| Protein Name | Protein Pals1Maguk P55 Subfamily Member 5Membrane Protein - Palmitoylated 5Protein Associated With Lin-7 1 |
| Database Links | Reactome: R-HSA-420029Reactome: R-HSA-9692912Reactome: R-HSA-9705677 |
| Cellular Localisation | Golgi ApparatusCell MembranePeripheral Membrane ProteinEndomembrane SystemCell JunctionTight JunctionAdherens JunctionCell ProjectionAxonPerikaryonApical Cell MembraneLocalized To The Tight Junctions Of Epithelial CellsLocalized To The Golgi Apparatus In T LymphocytesLocalized To A Subset Of Intracellular VesiclesLocalized To The Purkinje Cell Body And AxonLocalized To Intercellular Junctions In Vascular Endothelial CellsLocalized To Schmidt-Lanterman IncisuresThe Adaxonal DomainAnd The Inner Part Of Paranodal Loops In Myelinating Schwann Cells Of The Sciatic NerveLocalized To Apical Membrane Domains Of The Outer Limiting Membrane (Olm) Junctions In The RetinaColocalizes With Crb1 At The OlmApical To The Adherens JunctionColocalizes With Mpp1 In The Retina At The OlmColocalizes With Mpp3 To The Subapical Region Of Adherens Junctions In The Retina OlmEndoplasmic Reticulum-Golgi Intermediate Compartment(Microbial Infection) Following Infection By Human Sars CoronavirusPartially Localized At The Site Of Viral ReplicationThe Endoplasmic Reticulum-Golgi Intermediate CompartmentReducing Its Levels At Cell-Cell Contacts Which Results In Delayed Formation Of Tight Junctions And Affects Establishment Of Cell Polarity |
| Alternative Antibody Names | Anti-Protein Pals1 antibodyAnti-Maguk P55 Subfamily Member 5 antibodyAnti-Membrane Protein - Palmitoylated 5 antibodyAnti-Protein Associated With Lin-7 1 antibodyAnti-PALS1 antibodyAnti-MPP5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
