• Western blot analysis of various lysates using MIS12 Rabbit polyclonal antibody (STJ117703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MIS12 antibody (1-205) (STJ117703)

SKU:
STJ117703

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MIS12 (1-205) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIS12
Gene ID: 79003
Uniprot ID: MIS12_HUMAN
Immunogen Region: 1-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MIS12 (NP_076944.1).
Immunogen Sequence: MSVDPMTYEAQFFGFTPQTC MLRIYIAFQDYLFEVMQAVE QVILKKLDGIPDCDISPVQI RKCTEKFLCFMKGHFDNLFS KMEQLFLQLILRIPSNILLP EDKCKETPYSEEDFQHLQKE IEQLQEKYKTELCTKQALLA ELEEQKIVQAKLKQTLTFFD ELHNVGRDHGTSDFRESLVS LVQNSRKLQNIRDNVEKESK RLKIS
Function Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.
Protein Name Protein Mis12 Homolog
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Chromosome
Centromere
Kinetochore
Associated With The Kinetochore
Alternative Antibody Names Anti-Protein Mis12 Homolog antibody
Anti-MIS12 antibody

Information sourced from Uniprot.org

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