• Western blot analysis of extracts of various cell lines, using MID2 antibody (STJ110623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
  • Western blot analysis of extracts of various cell lines, using MID2 antibody (STJ110623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-MID2 antibody (476-705) (STJ110623)

SKU:
STJ110623

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MID2 (476-705) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MID2
Gene ID: 11043
Uniprot ID: TRIM1_HUMAN
Immunogen Region: 476-705
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 476-705 of human MID2 (NP_438112.2).
Immunogen Sequence: GTRYIFIVKAINQAGSRNSE PTRLKTNSQPFKLDPKMTHK KLKISNDGLQMEKDESSLKK SHTPERFSGTGCYGAAGNIF IDSGCHYWEVVMGSSTWYAI GIAYKSAPKNEWIGKNASSW VFSRCNSNFVVRHNNKEMLV DVPPHLKRLGVLLDYDNNML SFYDPANSLHLHTFDVTFIL PVCPTFTIWNKSLMILSGLP APDFIDYPERQECNCRPQES PYVSGMKTCH
Tissue Specificity Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
Post Translational Modifications Phosphorylated on serine and threonine residues.
Function E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29.
Protein Name Probable E3 Ubiquitin-Protein Ligase Mid2
Midin-2
Midline Defect 2
Midline-2
Ring Finger Protein 60
Ring-Type E3 Ubiquitin Transferase Mid2
Tripartite Motif-Containing Protein 1
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule-Associated
Alternative Antibody Names Anti-Probable E3 Ubiquitin-Protein Ligase Mid2 antibody
Anti-Midin-2 antibody
Anti-Midline Defect 2 antibody
Anti-Midline-2 antibody
Anti-Ring Finger Protein 60 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mid2 antibody
Anti-Tripartite Motif-Containing Protein 1 antibody
Anti-MID2 antibody
Anti-FXY2 antibody
Anti-RNF60 antibody
Anti-TRIM1 antibody

Information sourced from Uniprot.org

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