• Western blot analysis of extracts of various cell lines, using IYD antibody (STJ111610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IYD antibody (24-214) (STJ111610)

SKU:
STJ111610

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IYD (24-214) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IYD
Gene ID: 389434
Uniprot ID: IYD1_HUMAN
Immunogen Region: 24-214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-214 of human IYD (NP_981932.1).
Immunogen Sequence: DRSMEKKKGEPRTRAEARPW VDEDLKDSSDLHQAEEDADE WQESEENVEHIPFSHNHYPE KEMVKRSQEFYELLNKRRSV RFISNEQVPMEVIDNVIRTA GTAPSGAHTEPWTFVVVKDP DVKHKIRKIIEEEEEINYMK RMGHRWVTDLKKLRTNWIKE YLDTAPILILIFKQVHGFAA NGKKKVHYYNE
Tissue Specificity Expressed at a high level in thyroid gland (at protein level). Expressed at a high level in thyroid gland and at lower level in kidney and trachea.
Function Catalyzes the dehalogenation of halotyrosines such as 3-bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5-diiodo-L-tyrosine. During thyroid hormone biosynthesis, facilitates iodide salvage by catalysing the oxidative NADPH-dependent deiodination of the halogenated by-products of thyroid hormone production, monoiodotyrosine (L-MIT) and diiodotyrosine (L-DIT). The scavanged iodide can then reenter the hormone-producing pathways. Acts more efficiently on 3-iodo-L-tyrosine than 3,5-diiodo-L-tyrosine.
Protein Name Iodotyrosine Deiodinase 1
Iyd-1
Iodotyrosine Dehalogenase 1
Database Links Reactome: R-HSA-209968
Cellular Localisation Cell Membrane
Single-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Alternative Antibody Names Anti-Iodotyrosine Deiodinase 1 antibody
Anti-Iyd-1 antibody
Anti-Iodotyrosine Dehalogenase 1 antibody
Anti-IYD antibody
Anti-C6orf71 antibody
Anti-DEHAL1 antibody

Information sourced from Uniprot.org

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