• Western blot analysis of lysates from HepG2 cells, using HNF1B Rabbit polyclonal antibody (STJ24057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using HNF1B Rabbit polyclonal antibody (STJ24057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HNF1B antibody (1-190) (STJ24057)

SKU:
STJ24057

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNF1B (1-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNF1B
Gene ID: 6928
Uniprot ID: HNF1B_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of HNF1B (NP_000449.1).
Immunogen Sequence: MVSKLTSLQQELLSALLSSG VTKEVLVQALEELLPSPNFG VKLETLPLSPGSGAEPDTKP VFHTLTNGHAKGRLSGDEGS EDGDDYDTPPILKELQALNT EEAAEQRAEVDRMLSEDPWR AAKMIKGYMQQHNIPQREVV DVTGLNQSHLSQHLNKGTPM KTQKRAALYTWYVRKQREIL RQFNQTVQSS
Function Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Binds to the FPC element in the cAMP regulatory unit of the PLAU gene. Transcriptional activity is increased by coactivator PCBD1.
Protein Name Hepatocyte Nuclear Factor 1-Beta
Hnf-1-Beta
Hnf-1b
Homeoprotein Lfb3
Transcription Factor 2
Tcf-2
Variant Hepatic Nuclear Factor 1
Vhnf1
Database Links Reactome: R-HSA-210744
Reactome: R-HSA-210747
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 1-Beta antibody
Anti-Hnf-1-Beta antibody
Anti-Hnf-1b antibody
Anti-Homeoprotein Lfb3 antibody
Anti-Transcription Factor 2 antibody
Anti-Tcf-2 antibody
Anti-Variant Hepatic Nuclear Factor 1 antibody
Anti-Vhnf1 antibody
Anti-HNF1B antibody
Anti-TCF2 antibody

Information sourced from Uniprot.org

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