• Immunohistochemistry analysis of HMGCS2 in paraffin-embedded human liver using HMGCS2 Rabbit monoclonal antibody (STJ11102964) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using HMGCS2 Rabbit monoclonal antibody (STJ11102964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using HMGCS2 Rabbit monoclonal antibody (STJ11102964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HMGCS2 antibody (409-508) [S4MR] (STJ11102964)

SKU:
STJ11102964

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HMGCS2 (409-508) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGCS2
Gene ID: 3158
Uniprot ID: HMCS2_HUMAN
Immunogen Region: 409-508
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 409-508 of human HMGCS2 (P54868).
Immunogen Sequence: AFSYGSGLAASFFSFRVSQD AAPGSPLDKLVSSTSDLPKR LASRKCVSPEEFTEIMNQRE QFYHKVNFSPPGDTNSLFPG TWYLERVDEQHRRKYARRPV
Tissue Specificity Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain. Isoform 3: Highest expression detected in heart and skeletal muscle.
Post Translational Modifications Succinylated. Desuccinylated by SIRT5. Succinylation, at least at Lys-83 and Lys-310, inhibits the enzymatic activity.
Function Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
Protein Name Hydroxymethylglutaryl-Coa Synthase - Mitochondrial
Hmg-Coa Synthase
3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-77111
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Hydroxymethylglutaryl-Coa Synthase - Mitochondrial antibody
Anti-Hmg-Coa Synthase antibody
Anti-3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase antibody
Anti-HMGCS2 antibody

Information sourced from Uniprot.org

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