• Immunohistochemistry analysis of paraffin-embedded mouse embryos using HMGA2 antibody (STJ24036) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HMGA2 antibody (STJ24036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using HMGA2 antibody (STJ24036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HMGA2 antibody (55-109) (STJ24036)

SKU:
STJ24036

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGA2
Gene ID: 8091
Uniprot ID: HMGA2_HUMAN
Immunogen Region: 55-109
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 55-109 of human HMGA2 (NP_003474.1).
Immunogen Sequence: SKNKSPSKAAQKKAEATGEK RPRGRPRKWPQQVVQKKPAQ EETEETSSQESAEED
Post Translational Modifications Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2.
Function Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation. Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner.
Protein Name High Mobility Group Protein Hmgi-C
High Mobility Group At-Hook Protein 2
Database Links Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Alternative Antibody Names Anti-High Mobility Group Protein Hmgi-C antibody
Anti-High Mobility Group At-Hook Protein 2 antibody
Anti-HMGA2 antibody
Anti-HMGIC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance