| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HHIP (400-700) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HHIP |
| Gene ID: |
64399 |
| Uniprot ID: |
HHIP_HUMAN |
| Immunogen Region: |
400-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HHIP (NP_071920.1). |
| Immunogen Sequence: |
DMCNVPYSIPRSNPHFNSTN QPPEVFAHGLHDPGRCAVDR HPTDININLTILCSDSNGKN RSSARILQIIKGKDYESEPS LLEFKPFSNGPLVGGFVYRG CQSERLYGSYVFGDRNGNFL TLQQSPVTKQWQEKPLCLGT SGSCRGYFSGHILGFGEDEL GEVYILSSSKSMTQTHNGKL YKIVDPKRPLMPEECRATVQ PAQTLTSECSRLCRNGYCTP TGKCCCSPGWEGDFCRTAK |
| Tissue Specificity | Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney. |
| Function | Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. |
| Protein Name | Hedgehog-Interacting ProteinHhipHip |
| Database Links | Reactome: R-HSA-5632681 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinSecretedThe Last 22 C-Terminal Amino Acids May Participate In Cell Membrane AttachmentIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Hedgehog-Interacting Protein antibodyAnti-Hhip antibodyAnti-Hip antibodyAnti-HHIP antibodyAnti-HIP antibodyAnti-UNQ5825 antibodyAnti-PRO19644 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89094/357553/STJ11100022_1__83889.1713121675.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")