• Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-HERC4 antibody (400-700) (STJ11100022)

SKU:
STJ11100022

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HERC4 (400-700) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HERC4
Gene ID: 26091
Uniprot ID: HERC4_HUMAN
Immunogen Region: 400-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2).
Immunogen Sequence: WTVNEALIQKWLSYPSGRFP VEIANEIDGTFSSSGCLNGS FLAVSNDDHYRTGTRFSGVD MNAARLLFHKLIQPDHPQIS QQVAASLEKNLIPKLTSSLP DVEALRFYLTLPECPLMSDS NNFTTIAIPFGTALVNLEKA PLKVLENWWSVLEPPLFLKI VELFKEVVVHLLKLYKIGIP PSERRIFNSFLHTALKVLEI LHRVNEKMGQIIQYDKFYIH EVQELIDIRNDYINWVQQQ
Tissue Specificity Expressed in brain and testis and detected in heart and placenta.
Function Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Protein Name Probable E3 Ubiquitin-Protein Ligase Herc4
Hect Domain And Rcc1-Like Domain-Containing Protein 4
Hect-Type E3 Ubiquitin Transferase Herc4
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytosol
Shows A Punctate Cytoplasmic Distribution
Alternative Antibody Names Anti-Probable E3 Ubiquitin-Protein Ligase Herc4 antibody
Anti-Hect Domain And Rcc1-Like Domain-Containing Protein 4 antibody
Anti-Hect-Type E3 Ubiquitin Transferase Herc4 antibody
Anti-HERC4 antibody
Anti-KIAA1593 antibody

Information sourced from Uniprot.org

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