• Immunofluorescence analysis of U2OS cells using GOLGB1 Rabbit polyclonal antibody (STJ11103313) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using GOLGB1 Rabbit polyclonal antibody (STJ11103313) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GOLGB1 Rabbit polyclonal antibody (STJ11103313) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HeLa cells, using GOLGB1 antibody (STJ11103313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GOLGB1 antibody (1704-1797) (STJ11103313)

SKU:
STJ11103313

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOLGB1 (1704-1797) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLGB1
Gene ID: 2804
Uniprot ID: GOGB1_HUMAN
Immunogen Region: 1704-1797
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1704-1797 of human GOLGB1 (NP_004478.3).
Immunogen Sequence: HPAGDTAKECMETLLSSNAS MKEELERVKMEYETLSKKFQ SLMSEKDSLSEEVQDLKHQI EGNVSKQANLEATEKHDNQT NVTEEGTQSIPGET
Function May participate in forming intercisternal cross-bridges of the Golgi complex.
Protein Name Golgin Subfamily B Member 1
372 Kda Golgi Complex-Associated Protein
Gcp372
Giantin
Macrogolgin
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-6807878
Reactome: R-HSA-9703465
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Golgin Subfamily B Member 1 antibody
Anti-372 Kda Golgi Complex-Associated Protein antibody
Anti-Gcp372 antibody
Anti-Giantin antibody
Anti-Macrogolgin antibody
Anti-GOLGB1 antibody

Information sourced from Uniprot.org

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