• Immunohistochemistry analysis of paraffin-embedded human stomach using GNAI3 antibody (STJ29871) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GNAI3 antibody (STJ29871) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GNAI3 antibody (STJ29871) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-GNAI3 antibody (1-100) (STJ29871)

SKU:
STJ29871

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNAI3 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAI3
Gene ID: 2773
Uniprot ID: GNAI3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).
Immunogen Sequence: MGCTLSAEDKAAVERSKMID RNLREDGEKAAKEVKLLLLG AGESGKSTIVKQMKIIHEDG YSEDECKQYKVVVYSNTIQS IIAIIRAMGRLKIDFGEAAR
Post Translational Modifications (Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Function Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Protein Name Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3
G(I Alpha-3
Database Links Reactome: R-HSA-170670
Reactome: R-HSA-392170
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-9009391
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Cellular Localisation Cytoplasm
Cell Membrane
Lipid-Anchor
Cytoskeleton
Microtubule Organizing Center
Centrosome
Localizes In The Centrosomes Of Interphase And Mitotic Cells
Detected At The Cleavage Furrow And/Or The Midbody
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3 antibody
Anti-G(I Alpha-3 antibody
Anti-GNAI3 antibody

Information sourced from Uniprot.org

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