• Immunofluorescence analysis of A549 cells using GBA3 antibody (STJ110137). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GBA3 antibody (STJ110137) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-GBA3 antibody (STJ110137)

SKU:
STJ110137
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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against GBA3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:100
Uniprot ID: GBA3_HUMAN
Gene ID: 57733
Gene Symbol: GBA3
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human GBA3 (NP_001121904.1).
Background The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Tissue Specificity Present in small intestine (at protein level). Expressed in liver, small intestine, colon, spleen and kidney. Down-regulated in renal cell carcinomas and hepatocellular carcinomas.
Post Translational Modifications The N-terminus is blocked.
Function Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides. Has a significant glucosylceramidase activity in vitro. However, that activity is relatively low and its significance in vivo is not clear. Also able to hydrolyze galactosylceramide/GalCer, glucosylsphingosine/GlcSph and galactosylsphingosine/GalSph. However, the in vivo relevance of these activities is unclear. It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics. Could also play a role in the catabolism of cytosolic sialyl free N-glycans.
Database Links Reactome: R-HSA-1660662
Cellular Localisation Cytoplasm
Cytosol
Protein Name Cytosolic Beta-Glucosidase
Cytosolic Beta-Glucosidase-Like Protein 1
Cytosolic Glycosylceramidase
Cytosolic Gcase
Glucosidase Beta Acid 3
Glucosylceramidase Beta 3
Klotho-Related Protein
Klrp
Alternative Names Anti-Cytosolic Beta-Glucosidase antibody
Anti-Cytosolic Beta-Glucosidase-Like Protein 1 antibody
Anti-Cytosolic Glycosylceramidase antibody
Anti-Cytosolic Gcase antibody
Anti-Glucosidase Beta Acid 3 antibody
Anti-Glucosylceramidase Beta 3 antibody
Anti-Klotho-Related Protein antibody
Anti-Klrp antibody
Anti-GBA3 antibody
Anti-CBG antibody
Anti-CBGL1 antibody

Information sourced from uniprot.org

6 months standard