| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FOS |
| Gene ID: | 2353 |
| Uniprot ID: | FOS_HUMAN |
| Immunogen Region: | 1-114 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1). |
| Immunogen Sequence: | MMFSGFNADYEASSSRCSSA SPAGDSLSYYHSPADSFSSM GSPVNAQDFCTDLAVSSANF IPTVTAISTSPDLQWLVQPA LVSSVAPSQTRAPHPFGVPA PSAGAYSRAGVVKT |
| Post Translational Modifications | Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation. Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis. |
| Function | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. |
| Protein Name | Protein C-Fos Cellular Oncogene Fos Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit G0/G1 Switch Regulatory Protein 7 Proto-Oncogene C-Fos Transcription Factor Ap-1 Subunit C-Fos |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2871796 Reactome: R-HSA-450341 Reactome: R-HSA-6785807 Reactome: R-HSA-6796648 Reactome: R-HSA-9018519 Reactome: R-HSA-9031628 Reactome: R-HSA-9634638 Reactome: R-HSA-9768919 |
| Cellular Localisation | Nucleus Endoplasmic Reticulum Cytoplasm Cytosol In Quiescent Cells Present In Very Small Amounts In The Cytosol Following Induction Of Cell Growth First Localizes To The Endoplasmic Reticulum And Only Later To The Nucleus Localization At The Endoplasmic Reticulum Requires Dephosphorylation At Tyr-10 And Tyr-30 |
| Alternative Antibody Names | Anti-Protein C-Fos antibody Anti-Cellular Oncogene Fos antibody Anti-Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit antibody Anti-G0/G1 Switch Regulatory Protein 7 antibody Anti-Proto-Oncogene C-Fos antibody Anti-Transcription Factor Ap-1 Subunit C-Fos antibody Anti-FOS antibody Anti-G0S7 antibody |
Information sourced from Uniprot.org