| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FEM1B (368-627) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FEM1B |
| Gene ID: |
10116 |
| Uniprot ID: |
FEM1B_HUMAN |
| Immunogen Region: |
368-627 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 368-627 of human FEM1B (NP_056137.1). |
| Immunogen Sequence: |
LHALHLRQKGNRNTHKDLLR FAQVFSQMIHLNETVKAPDI ECVLRCSVLEIEQSMNRVKN ISDADVHNAMDNYECNLYTF LYLVCISTKTQCSEEDQCKI NKQIYNLIHLDPRTREGFTL LHLAVNSNTPVDDFHTNDVC SFPNALVTKLLLDCGAEVNA VDNEGNSALHIIVQYNRPIS DFLTLHSIIISLVEAGAHTD MTNKQNKTPLDKSTTGVSEI LLKTQMKMSLKCLAARAVR |
| Tissue Specificity | Widely expressed. Highly expressed in testis. Weakly expressed in other tissues. |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation. Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS). Mechanistically, recognizes and binds reduced FNIP1 through two interface zinc ions, which act as a molecular glue that recruit reduced FNIP1 to FEM1B. Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity. Promotes ubiquitination and degradation of ANKRD37. Promotes ubiquitination and degradation of SLBP. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. |
| Protein Name | Protein Fem-1 Homolog BFem1bFem1-BetaFem-1-Like Death Receptor-Binding Protein AlphaFem-1-Like In Apoptotic Pathway Protein AlphaF1a-Alpha |
| Database Links | Reactome: R-HSA-8951664 |
| Cellular Localisation | CytoplasmNucleusIn The NucleusThe Protein Level Increased Slightly After Camptothecin (Cpt) TreatmentAssociated With Chromatin |
| Alternative Antibody Names | Anti-Protein Fem-1 Homolog B antibodyAnti-Fem1b antibodyAnti-Fem1-Beta antibodyAnti-Fem-1-Like Death Receptor-Binding Protein Alpha antibodyAnti-Fem-1-Like In Apoptotic Pathway Protein Alpha antibodyAnti-F1a-Alpha antibodyAnti-FEM1B antibodyAnti-F1AA antibodyAnti-KIAA0396 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
