| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FEM1B |
| Gene ID: | 10116 |
| Uniprot ID: | FEM1B_HUMAN |
| Immunogen Region: | 368-627 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 368-627 of human FEM1B (NP_056137.1). |
| Immunogen Sequence: | LHALHLRQKGNRNTHKDLLR FAQVFSQMIHLNETVKAPDI ECVLRCSVLEIEQSMNRVKN ISDADVHNAMDNYECNLYTF LYLVCISTKTQCSEEDQCKI NKQIYNLIHLDPRTREGFTL LHLAVNSNTPVDDFHTNDVC SFPNALVTKLLLDCGAEVNA VDNEGNSALHIIVQYNRPIS DFLTLHSIIISLVEAGAHTD MTNKQNKTPLDKSTTGVSEI LLKTQMKMSLKCLAARAVR |
| Tissue Specificity | Widely expressed. Highly expressed in testis. Weakly expressed in other tissues. |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation. Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS). Mechanistically, recognizes and binds reduced FNIP1 through two interface zinc ions, which act as a molecular glue that recruit reduced FNIP1 to FEM1B. Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity. Promotes ubiquitination and degradation of ANKRD37. Promotes ubiquitination and degradation of SLBP. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. |
| Protein Name | Protein Fem-1 Homolog B Fem1b Fem1-Beta Fem-1-Like Death Receptor-Binding Protein Alpha Fem-1-Like In Apoptotic Pathway Protein Alpha F1a-Alpha |
| Database Links | Reactome: R-HSA-8951664 |
| Cellular Localisation | Cytoplasm Nucleus In The Nucleus The Protein Level Increased Slightly After Camptothecin (Cpt) Treatment Associated With Chromatin |
| Alternative Antibody Names | Anti-Protein Fem-1 Homolog B antibody Anti-Fem1b antibody Anti-Fem1-Beta antibody Anti-Fem-1-Like Death Receptor-Binding Protein Alpha antibody Anti-Fem-1-Like In Apoptotic Pathway Protein Alpha antibody Anti-F1a-Alpha antibody Anti-FEM1B antibody Anti-F1AA antibody Anti-KIAA0396 antibody |
Information sourced from Uniprot.org