• Western blot analysis of extracts of various cell lines, using FABPI rabbit monoclonal antibody (STJ11102391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Confocal imaging of paraffin-embedded mouse small intestine using FABPI Rabbit monoclonal antibody (STJ11102391, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded rat intestine using FABPI rabbit monoclonal antibody (STJ11102391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FABPI in paraffin-embedded mouse intestin using FABPI Rabbit monoclonal antibody (STJ11102391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse intestin using FABPI rabbit monoclonal antibody (STJ11102391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FABPI in paraffin-embedded rat intestine using FABPI Rabbit monoclonal antibody (STJ11102391) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse intestin using FABPI rabbit monoclonal antibody (STJ11102391) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using FABPI Rabbit monoclonal antibody (STJ11102391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FABP2 antibody (1-100) [S1MR] (STJ11102391)

SKU:
STJ11102391

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FABPI (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FABP2
Gene ID: 2169
Uniprot ID: FABPI_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FABPI (P12104).
Immunogen Sequence: MAFDSTWKVDRSENYDKFME KMGVNIVKRKLAAHDNLKLT ITQEGNKFTVKESSAFRNIE VVFELGVTFNYNLADGTELR GTWSLEGNKLIGKFKRTDNG
Tissue Specificity Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
Function FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Protein Name Fatty Acid-Binding Protein - Intestinal
Fatty Acid-Binding Protein 2
Intestinal-Type Fatty Acid-Binding Protein
I-Fabp
Database Links Reactome: R-HSA-163560
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Fatty Acid-Binding Protein - Intestinal antibody
Anti-Fatty Acid-Binding Protein 2 antibody
Anti-Intestinal-Type Fatty Acid-Binding Protein antibody
Anti-I-Fabp antibody
Anti-FABP2 antibody
Anti-FABPI antibody

Information sourced from Uniprot.org

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