• Western blot analysis of extracts of various cell lines, using Coagulation factor III/Coagulation factor III/Tissue Factor rabbit monoclonal antibody (STJ11102068) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-F3 antibody (1-100) [S8MR] (STJ11102068)

SKU:
STJ11102068

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Tissue Factor (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F3
Gene ID: 2152
Uniprot ID: TF_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Coagulation factor III/Tissue Factor (P13726).
Immunogen Sequence: METPAWPRVPRPETAVARTL LLGWVFAQVAGASGTTNTVA AYNLTWKSTNFKTILEWEPK PVNQVYTVQISTKSGDWKSK CFYTTDTECDLTDEIVKDVK
Tissue Specificity Lung, placenta and pancreas.
Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Protein Name Tissue Factor
Tf
Coagulation Factor Iii
Thromboplastin
Cd Antigen Cd142
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-9031628
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Tissue Factor antibody
Anti-Tf antibody
Anti-Coagulation Factor Iii antibody
Anti-Thromboplastin antibody
Anti-Cd Antigen Cd142 antibody
Anti-F3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance