| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EXT2 |
| Gene ID: |
2132 |
| Uniprot ID: |
EXT2_HUMAN |
| Immunogen Region: |
80-340 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EXT2 (NP_000392.3). |
| Immunogen Sequence: |
PHSIESSNDWNVEKRSIRDV PVVRLPADSPIPERGDLSCR MHTCFDVYRCGFNPKNKIKV YIYALKKYVDDFGVSVSNTI SREYNELLMAISDSDYYTDD INRACLFVPSIDVLNQNTLR IKETAQAMAQLSRWDRGTNH LLFNMLPGGPPDYNTALDVP RDRALLAGGGFSTWTYRQGY DVSIPVYSPLSAEVDLPEKG PGPRQYFLLSSQVGLHPEYR EDLEALQVKHGESVLVLDK |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | N-glycosylated at Asn-637. A soluble form is generated by proteolytic processing. |
| Function | Glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone. Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity. Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling. |
| Protein Name | Exostosin-2Exostosin Glycosyltransferase 2Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-AcetylglucosaminyltransferaseHeparan Sulfate Co-Polymerase Subunit Ext1Multiple Exostoses Protein 2 |
| Database Links | Reactome: R-HSA-2022928Reactome: R-HSA-3656237Reactome: R-HSA-3656253 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinGolgi ApparatusCis-Golgi Network MembraneEndoplasmic Reticulum MembraneSecretedThe Active Heparan Sulfate Polymerase Complex Composed Of Ext1 And Ext2 Is Localized To The Golgi ApparatusIf Both Proteins Are Individually Detected In The Endoplasmic ReticulumThe Formation Of The Complex Promotes Their Transport To The Golgi |
| Alternative Antibody Names | Anti-Exostosin-2 antibodyAnti-Exostosin Glycosyltransferase 2 antibodyAnti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibodyAnti-Heparan Sulfate Co-Polymerase Subunit Ext1 antibodyAnti-Multiple Exostoses Protein 2 antibodyAnti-EXT2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98784/382834/STJ119999_1__50326.1713151342.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")