• Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF3H antibody (80-340) (STJ119999)

SKU:
STJ119999

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF3H (80-340) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF3H
Gene ID: 8667
Uniprot ID: EIF3H_HUMAN
Immunogen Region: 80-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Immunogen Sequence: NCFPFPQHTEDDADFDEVQY QMEMMRSLRHVNIDHLHVGW YQSTYYGSFVTRALLDSQFS YQHAIEESVVLIYDPIKTAQ GSLSLKAYRLTPKLMEVCKE KDFSPEALKKANITFEYMFE EVPIVIKNSHLINVLMWELE KKSAVADKHELLSLASSNHL GKNLQLLMDRVDEMSQDIVK YNTYMRNTSKQQQQKHQYQQ RRQQENMQRQSRGEPPLPEE DLSKLFKPPQPPARMDSLL
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
Protein Name Eukaryotic Translation Initiation Factor 3 Subunit H
Eif3h
Eukaryotic Translation Initiation Factor 3 Subunit 3
Eif-3-Gamma
Eif3 P40 Subunit
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 3 Subunit H antibody
Anti-Eif3h antibody
Anti-Eukaryotic Translation Initiation Factor 3 Subunit 3 antibody
Anti-Eif-3-Gamma antibody
Anti-Eif3 P40 Subunit antibody
Anti-EIF3H antibody
Anti-EIF3S3 antibody

Information sourced from Uniprot.org

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