• Immunofluorescence analysis of A-431 cells using Engrailed 1 Rabbit polyclonal antibody (STJ11105000) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EN1 antibody (STJ11105000)

SKU:
STJ11105000

Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Homeobox Protein Engrailed-1 is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EN1
Gene ID: 2019
Uniprot ID: HME1_HUMAN
Immunogen: Recombinant protein of human Engrailed 1.
Immunogen Sequence: KLKKKKNEKEDKRPRTAFTA EQLQRLKAEFQANRYITEQR RQTLAQELSLNESQIKIWFQ NKRAKIKKATGIKNGLALHL MAQGLYNHSTTTVQDKDESE
Function Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb.
Protein Name Homeobox Protein Engrailed-1
Homeobox Protein En-1
Hu-En-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Engrailed-1 antibody
Anti-Homeobox Protein En-1 antibody
Anti-Hu-En-1 antibody
Anti-EN1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance