| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EMG1 (1-244) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EMG1 |
| Gene ID: |
10436 |
| Uniprot ID: |
NEP1_HUMAN |
| Immunogen Region: |
1-244 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human EMG1 (NP_006322.4). |
| Immunogen Sequence: |
MAAPSDGFKPRERSGGEQAQ DWDALPPKRPRLGAGNKIGG RRLIVVLEGASLETVKVGKT YELLNCDKHKSILLKNGRDP GEARPDITHQSLLMLMDSPL NRAGLLQVYIHTQKNVLIEV NPQTRIPRTFDRFCGLMVQL LHKLSVRAADGPQKLLKVIK NPVSDHFPVGCMKVGTSFSI PVVSDVRELVPSSDPIVFVV GAFAHGKVSVEYTEKMVSIS NYPLSAALTCAKLTTAFEE |
| Function | S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. |
| Protein Name | Ribosomal Rna Small Subunit Methyltransferase Nep118s RrnaPseudouridine(1248-N1-Methyltransferase18s Rrna Psi1248 MethyltransferaseNucleolar Protein Emg1 HomologProtein C2fRibosome Biogenesis Protein Nep1 |
| Database Links | Reactome: R-HSA-6790901Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Ribosomal Rna Small Subunit Methyltransferase Nep1 antibodyAnti-18s Rrna antibodyAnti-Pseudouridine(1248-N1-Methyltransferase antibodyAnti-18s Rrna Psi1248 Methyltransferase antibodyAnti-Nucleolar Protein Emg1 Homolog antibodyAnti-Protein C2f antibodyAnti-Ribosome Biogenesis Protein Nep1 antibodyAnti-EMG1 antibodyAnti-C2F antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LRRC59 knockout (KO) 293T cells, using [KO Validated] LRRC59 Rabbit polyclonal antibody (STJ11100841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89775/358247/STJ11100841_1__11256.1713122582.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LRRC59 knockout (KO) 293T cells, using [KO Validated] LRRC59 Rabbit polyclonal antibody (STJ11100841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
