| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF4G2 (320-490) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF4G2 |
| Gene ID: |
1982 |
| Uniprot ID: |
IF4G2_HUMAN |
| Immunogen Region: |
320-490 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 320-490 of human EIF4G2/p97 (NP_001409.3). |
| Immunogen Sequence: |
PKTINQIRQDAVKDLGVFIP APMAQGMRSDFFLEGPFMPP RMKMDRDPLGGLADMFGQMP GSGIGTGPGVIQDRFSPTMG RHRSNQLFNGHGGHIMPPTQ SQFGEMGGKFMKSQGLSQLY HNQSQGLLSQLQGQSKDMPP RFSKKGQLNADEISLRPAQS FLMNKNQVPKL |
| Tissue Specificity | Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney. |
| Post Translational Modifications | Phosphorylation.hyperphosphorylated during mitosis. |
| Function | Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. |
| Protein Name | Eukaryotic Translation Initiation Factor 4 Gamma 2Eif-4-Gamma 2Eif-4g 2Eif4g 2Death-Associated Protein 5Dap-5P97 |
| Database Links | Reactome: R-HSA-1169408 |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4 Gamma 2 antibodyAnti-Eif-4-Gamma 2 antibodyAnti-Eif-4g 2 antibodyAnti-Eif4g 2 antibodyAnti-Death-Associated Protein 5 antibodyAnti-Dap-5 antibodyAnti-P97 antibodyAnti-EIF4G2 antibodyAnti-DAP5 antibodyAnti-OK antibodyAnti-SW-cl.75 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and EIF4G2/p97 knockout (KO) 293T cells, using [KO Validated] EIF4G2/p97 Rabbit polyclonal antibody (STJ11100866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89800/358268/STJ11100866_1__08358.1713122610.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and EIF4G2/p97 knockout (KO) 293T cells, using [KO Validated] EIF4G2/p97 Rabbit polyclonal antibody (STJ11100866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
