• Western blot analysis of extracts of various cell lines, using DDX5 rabbit monoclonal antibody (STJ11101314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded human cervix cancer tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded human thyroid cancer tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded human tonsil tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse brain tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse colon tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse kidney tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse lung tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse spleen tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded mouse testis tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded rat brain tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DDX5 in paraffin-embedded rat colon tissue using DDX5 rabbit monoclonal antibody (STJ11101314) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using DDX5 rabbit monoclonal antibody (STJ11101314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using DDX5 rabbit monoclonal antibody (STJ11101314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DDX5 rabbit monoclonal antibody (STJ11101314) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse testis using 3 Mu g DDX5 antibody (STJ11101314). Western blot was performed from the immunoprecipitate using DDX5 (STJ11101314) at a dilution of 1:1000.

Anti-DDX5 antibody (1-100) [S4MR] (STJ11101314)

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STJ11101314

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DDX5 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX5
Gene ID: 1655
Uniprot ID: DDX5_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDX5 (P17844).
Immunogen Sequence: MSGYSSDRDRGRDRGFGAPR FGGSRAGPLSGKKFGNPGEK LVKKKWNLDELPKFEKNFYQ EHPDLARRTAQEVETYRRSK EITVRGHNCPKPVLNFYEAN
Post Translational Modifications Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Sumoylated.sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. Polyubiquitinated, leading to proteasomal degradation. Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues.
Function Involved in the alternative regulation of pre-mRNA splicing.its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation.the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner.the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
Protein Name Probable Atp-Dependent Rna Helicase Ddx5
Dead Box Protein 5
Rna Helicase P68
Database Links Reactome: R-HSA-3899300
Reactome: R-HSA-72163
Reactome: R-HSA-9018519
Reactome: R-HSA-9682706
Reactome: R-HSA-9694686
Cellular Localisation Nucleus
Nucleolus
Nucleus Speckle
Cytoplasm
During The G0 Phase
Predominantly Located In The Nucleus
Cytoplasmic Levels Increase During The G1/S Phase
During The M Phase
Located At The Vicinity Of The Condensed Chromosomes
At G1
Localizes In The Cytoplasm
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx5 antibody
Anti-Dead Box Protein 5 antibody
Anti-Rna Helicase P68 antibody
Anti-DDX5 antibody
Anti-G17P1 antibody
Anti-HELR antibody
Anti-HLR1 antibody

Information sourced from Uniprot.org

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