• Immunofluorescence analysis of paraffin-embedded mouse brain using CNPase Rabbit monoclonal antibody (STJ11101571) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat brain using CNPase Rabbit monoclonal antibody (STJ11101571) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CNPase in paraffin-embedded human brain tissue using CNPase Rabbit monoclonal antibody (STJ11101571) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CNPase in paraffin-embedded rat brain tissue using CNPase Rabbit monoclonal antibody (STJ11101571) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CNPase in paraffin-embedded mouse brain tissue using CNPase Rabbit monoclonal antibody (STJ11101571) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CNPase Rabbit monoclonal antibody (STJ11101571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CNP antibody (322-421) [S1MR] (STJ11101571)

SKU:
STJ11101571

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CNPase (322-421) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNP
Gene ID: 1267
Uniprot ID: CN37_HUMAN
Immunogen Region: 322-421
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 322-421 of human CNPase (P09543).
Immunogen Sequence: NLPRGSRAHITLGCAADVEA VQTGLDLLEILRQEKGGSRG EEVGELSRGKLYSLGNGRWM LTLAKNMEVRAIFTGYYGKG KPVPTQGSRKGGALQSCTII
Function Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates. May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.
Protein Name 2' -3'-Cyclic-Nucleotide 3'-Phosphodiesterase
Cnp
Cnpase
Cellular Localisation Membrane
Lipid-Anchor
Melanosome
Firmly Bound To Membrane Structures Of Brain White Matter
Alternative Antibody Names Anti-2' -3'-Cyclic-Nucleotide 3'-Phosphodiesterase antibody
Anti-Cnp antibody
Anti-Cnpase antibody
Anti-CNP antibody

Information sourced from Uniprot.org

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