• Western blot analysis of lysates from wild type (WT) and CNPasease knockout (KO) HeLa cells, using [KO Validated] CNPase Rabbit polyclonal antibody (STJ119982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CNP antibody (152-421) (STJ119982)

SKU:
STJ119982

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNP (152-421) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNP
Gene ID: 1267
Uniprot ID: CN37_HUMAN
Immunogen Region: 152-421
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 152-421 of human CNPasease (NP_149124.3).
Immunogen Sequence: TAWRLDCAQLKEKNQWQLSA DDLKKLKPGLEKDFLPLYFG WFLTKKSSETLRKAGQVFLE ELGNHKAFKKELRQFVPGDE PREKMDLVTYFGKRPPGVLH CTTKFCDYGKAPGAEEYAQQ DVLKKSYSKAFTLTISALFV TPKTTGARVELSEQQLQLWP SDVDKLSPTDNLPRGSRAHI TLGCAADVEAVQTGLDLLEI LRQEKGGSRGEEVGELSRGK LYSLGNGRWMLTLAKNMEV
Function Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates. May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.
Protein Name 2' -3'-Cyclic-Nucleotide 3'-Phosphodiesterase
Cnp
Cnpase
Cellular Localisation Membrane
Lipid-Anchor
Melanosome
Firmly Bound To Membrane Structures Of Brain White Matter
Alternative Antibody Names Anti-2' -3'-Cyclic-Nucleotide 3'-Phosphodiesterase antibody
Anti-Cnp antibody
Anti-Cnpase antibody
Anti-CNP antibody

Information sourced from Uniprot.org

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