• Western blot analysis of extracts of Jurkat cells, using CENPQ antibody (STJ29765) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CENPQ antibody (131-268) (STJ29765)

SKU:
STJ29765

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CENPQ (131-268) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPQ
Gene ID: 55166
Uniprot ID: CENPQ_HUMAN
Immunogen Region: 131-268
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 131-268 of human CENPQ (NP_060602.2).
Immunogen Sequence: MEDLTNVSSLLNMERARDKA NEEGLALLQEEIDKMVETTE LMTGNIQSLKNKIQILASEV EEEEERVKQMHQINSSGVLS LPELSQKTLKAPTLQKEILA LIPNQNALLKDLDILHNSSQ MKSMSTFIEEAYKKLDAS
Post Translational Modifications Phosphorylation at Ser-50 is essential for CENPE recruitment to kinetochores and orderly chromosome congression.
Function Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores.
Protein Name Centromere Protein Q
Cenp-Q
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-606279
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Localizes Exclusively In The Centromeres
The Cenpa-Cad Complex Is Probably Recruited On Centromeres By The Cenpa-Nac Complex
Alternative Antibody Names Anti-Centromere Protein Q antibody
Anti-Cenp-Q antibody
Anti-CENPQ antibody
Anti-C6orf139 antibody

Information sourced from Uniprot.org

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