• Western blot analysis of various lysates, using CD98 antibody (STJ11104992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using CD98 antibody (STJ11104992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD98 antibody (STJ11104992)

SKU:
STJ11104992

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD98 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Slc3a2
Gene ID: 17254
Uniprot ID: 4F2_MOUSE
Immunogen: Recombinant protein of mouse CD98.
Tissue Specificity Detected on the surface of embryonic epithelial cells in the epidermis, thymus, kidney, intestine, brain choroid plexus, and in retina. Detected in adult and embryonic brain, spleen, kidney, intestine and liver, and in adult testis (at protein level). Observed in all adult tissues tested with strongest expression in kidney, small intestine, spleen, thymus and liver. Moderate expression in brain, stomach, heart, testis, lung, skin, pancreas and skeletal muscle. In brain expressed on capillary endothelia in cerebral cortex.
Post Translational Modifications Phosphorylation on Ser-300 or Ser-302 and on Ser-420 by ecto-protein kinases favors heterotypic cell-cell interactions. N-glycosylated.N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane.
Function Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane. SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA. SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression.
Protein Name Amino Acid Transporter Heavy Chain Slc3a2
4f2 Cell-Surface Antigen Heavy Chain
4f2hc
Solute Carrier Family 3 Member 2
Cd Antigen Cd98
Database Links Reactome: R-MMU-352230
Reactome: -MMU-71240
Cellular Localisation Apical Cell Membrane
Cell Membrane
Single-Pass Type Ii Membrane Protein
Cell Junction
Lysosome Membrane
Melanosome
Basolateral Cell Membrane
Localized At The Plasma Membrane When Associated With Slc7a5 Or Slc7a8
Localized To The Apical Membrane Of Placental Syncytiotrophoblastic Cells
Recruited To Lysosomes By Laptm4b
Located Selectively At Cell-Cell Adhesion Sites
Colocalized With Slc7a8/Lat2 At The Basolateral Membrane Of Kidney Proximal Tubules And Small Intestine Epithelia
Expressed In Both Luminal And Abluminal Membranes Of Brain Capillary Endothelial Cells
Alternative Antibody Names Anti-Amino Acid Transporter Heavy Chain Slc3a2 antibody
Anti-4f2 Cell-Surface Antigen Heavy Chain antibody
Anti-4f2hc antibody
Anti-Solute Carrier Family 3 Member 2 antibody
Anti-Cd Antigen Cd98 antibody
Anti-Slc3a2 antibody
Anti-Mdu1 antibody

Information sourced from Uniprot.org

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