• Flow cytometry:1X10^6 Jurkat cells (negative control, left) and U-87MG cells (right) were surface-stained with CD68 Rabbit monoclonal antibody (STJ11103461, 2. 5 Mu g/mL, orange line) or Rabbit IgG isotype control ( 10 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
  • Confocal imaging of paraffin-embedded Human tonsil tissue using CD68 Rabbit monoclonal antibody (STJ11103461, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using CD68 Rabbit monoclonal antibody (STJ11103461) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using CD68 Rabbit monoclonal antibody (STJ11103461) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD68 antibody (100-200) [S1MR] (STJ11103461)

SKU:
STJ11103461

£146.50 - £639.50
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Host: Rabbit
Applications: WB/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD68 (100-200) is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD68
Gene ID: 968
Uniprot ID: CD68_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD68 (NP_001242.2).
Immunogen Sequence: TSQGPSTATHSPATTSHGNA TVHPTSNSTATSPGFTSSAH PEPPPPSPSPSPTSKETIGD YTWTNGSQPCVHLQAQIQIR VMYTTQGGGEAWGISVLNPN K
Tissue Specificity Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Post Translational Modifications N- and O-glycosylated.
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Protein Name Macrosialin
Gp110
Cd Antigen Cd68
Database Links Reactome: R-HSA-6798695
Cellular Localisation Isoform Short: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform Long: Endosome Membrane
Lysosome Membrane
Alternative Antibody Names Anti-Macrosialin antibody
Anti-Gp110 antibody
Anti-Cd Antigen Cd68 antibody
Anti-CD68 antibody

Information sourced from Uniprot.org

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