• Western blot analysis of extracts of various cell lines, using BUB3 rabbit monoclonal antibody (STJ11102383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U-2 OS cells using BUB3 Rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using BUB3 rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using BUB3 Rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using BUB3 rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded mouse lung tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using BUB3 rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded human colon tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using BUB3 rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded rat colon tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using BUB3 rabbit monoclonal antibody (STJ11102383) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded rat brain tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded mouse brain tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded human colon carcinoma tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of BUB3 in paraffin-embedded rat spleen tissue using BUB3 Rabbit monoclonal antibody (STJ11102383) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using BUB3 Rabbit monoclonal antibody (STJ11102383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BUB3 antibody (1-100) [S3MR] (STJ11102383)

SKU:
STJ11102383

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Bub3 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BUB3
Gene ID: 9184
Uniprot ID: BUB3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684).
Immunogen Sequence: MTGSNEFKLNQPPEDGISSV KFSPNTSQFLLVSSWDTSVR LYDVPANSMRLKYQHTGAVL DCAFYDPTHAWSGGLDHQLK MHDLNTDQENLVGTHDAPIR
Post Translational Modifications Poly-ADP-ribosylated by PARP1.
Function Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Protein Name Mitotic Checkpoint Protein Bub3
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-141444
Reactome: R-HSA-174184
Reactome: R-HSA-176409
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Starts To Localize At Kinetochores In Prometaphase I (Pro-Mi) Stage And Maintains The Localization Until The Metaphase I-Anaphase I (Mi-Ai) Transition
Alternative Antibody Names Anti-Mitotic Checkpoint Protein Bub3 antibody
Anti-BUB3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance