• Western blot analysis of extracts of various cell lines, using ARSF antibody (STJ110401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARSF antibody (1-200) (STJ110401)

SKU:
STJ110401

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARSF (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARSF
Gene ID: 416
Uniprot ID: ARSF_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ARSF (NP_004033.2).
Immunogen Sequence: MRPRRPLVFMSLVCALLNTC QAHRVHDDKPNIVLIMVDDL GIGDLGCYGNDTMRTPHIDR LAREGVRLTQHISAASLCSP SRSAFLTGRYPIRSGMVSSG NRRVIQNLAVPAGLPLNETT LAALLKKQGYSTGLIGKWHQ GLNCDSRSDQCHHPYNYGFD YYYGMPFTLVDSCWPDPSRN TELAFESQLWLCVQLVAIAI
Post Translational Modifications The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Function Exhibits arylsulfatase activity towards the artificial substrate 4-methylumbelliferyl sulfate.
Protein Name Arylsulfatase F
Asf
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-1663150
Cellular Localisation Secreted
Alternative Antibody Names Anti-Arylsulfatase F antibody
Anti-Asf antibody
Anti-ARSF antibody

Information sourced from Uniprot.org

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