• Western blot analysis of extracts of 293T cells, using ARRDC3 antibody (STJ114152) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARRDC3 antibody (1-270) (STJ114152)

SKU:
STJ114152

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARRDC3 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARRDC3
Gene ID: 57561
Uniprot ID: ARRD3_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ARRDC3 (NP_065852.1).
Immunogen Sequence: MVLGKVKSLTISFDCLNDSN VPVYSSGDTVSGRVNLEVTG EIRVKSLKIHARGHAKVRWT ESRNAGSNTAYTQNYTEEVE YFNHKDILIGHERDDDNSEE GFHTIHSGRHEYAFSFELPQ TPLATSFEGRHGSVRYWVKA ELHRPWLLPVKLKKEFTVFE HIDINTPSLLSPQAGTKEKT LCCWFCTSGPISLSAKIERK GYTPGESIQIFAEIENCSSR MVVPKAAIYQTQAFYAKGK
Tissue Specificity Highly expressed in skeletal muscle, placenta, kidney, lung, liver, blood, adrenal gland, lymph node, mammary gland, thyroid, and trachea. Very low levels in colon, thymus, spleen, small intestine, bladder and bone marrow. Strong expression in differentiated adipocytes compared to preadipocytes. Detected in omental fat and subcutaneous fat tissue.
Function Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.
Protein Name Arrestin Domain-Containing Protein 3
Tbp-2-Like Inducible Membrane Protein
Tlimp
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Lysosome
Endosome
Early Endosome
Associated With Plasma Membrane
As Well As With Endosomes And Lysosomes During Endocytosis
Alternative Antibody Names Anti-Arrestin Domain-Containing Protein 3 antibody
Anti-Tbp-2-Like Inducible Membrane Protein antibody
Anti-Tlimp antibody
Anti-ARRDC3 antibody
Anti-KIAA1376 antibody

Information sourced from Uniprot.org

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