• Western blot analysis of extracts of various cell lines, using ANKRD2 antibody (STJ22614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ANKRD2 antibody (30-140) (STJ22614)

SKU:
STJ22614

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKRD2 (30-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKRD2
Gene ID: 26287
Uniprot ID: ANKR2_HUMAN
Immunogen Region: 30-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-140 of human ANKRD2 (NP_001123453.1).
Immunogen Sequence: GTMEDSEAVQRATALIEQRL AQEEENEKLRGDARQKLPMD LLVLEDEKHHGAQSAALQKV KGQERVRKTSLDLRREIIDV GGIQNLIELRKKRKQKKRDA LAASHEPPPEP
Tissue Specificity Mostly expressed in skeletal and cardiac muscles. Found in slow fibers. Also expressed in kidney, but to a lower extent (at protein level).
Post Translational Modifications Phosphorylation at Ser-99 by PKB/AKT2 in response to oxidative stress induces translocation to the nucleus and negatively regulates myoblast differentiation.
Function Functions as a negative regulator of myocyte differentiation. May interact with both sarcoplasmic structural proteins and nuclear proteins to regulate gene expression during muscle development and in response to muscle stress.
Protein Name Ankyrin Repeat Domain-Containing Protein 2
Skeletal Muscle Ankyrin Repeat Protein
Harpp
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
I Band
Cytosol
Nucleus
Pml Body
In The Sarcoplasm Of Differentiated Striated Muscle Cells
Where It Is Cytosolic And Enriched In The I Band
In Nucleus And Pml Bodies Of Proliferating And Undifferentiated Myoblasts
Associates With The Euchromatin In The Nucleus Of Myocytes Upon Muscle Stress
Alternative Antibody Names Anti-Ankyrin Repeat Domain-Containing Protein 2 antibody
Anti-Skeletal Muscle Ankyrin Repeat Protein antibody
Anti-Harpp antibody
Anti-ANKRD2 antibody
Anti-ARPP antibody

Information sourced from Uniprot.org

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