• Western blot analysis of extracts of various cell lines, using ANAPC10 antibody (STJ110628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using ANAPC10 antibody (STJ110628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ANAPC10 antibody (1-185) (STJ110628)

SKU:
STJ110628

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANAPC10 (1-185) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANAPC10
Gene ID: 10393
Uniprot ID: APC10_HUMAN
Immunogen Region: 1-185
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human ANAPC10 (NP_055700.2).
Immunogen Sequence: MTTPNKTPPGADPKQLERTG TVREIGSQAVWSLSSCKPGF GVDQLRDDNLETYWQSDGSQ PHLVNIQFRRKTTVKTLCIY ADYKSDESYTPSKISVRVGN NFHNLQEIRQLELVEPSGWI HVPLTDNHKKPTRTFMIQIA VLANHQNGRDTHMRQIKIYT PVEESSIGKFPRCTTIDFMM YRSIR
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein Name Anaphase-Promoting Complex Subunit 10
Apc10
Cyclosome Subunit 10
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-174048
Reactome: R-HSA-174084
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-176407
Reactome: R-HSA-176408
Reactome: R-HSA-176409
Reactome: R-HSA-176412
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2559582
Reactome: R-HSA-68867
Reactome: R-HSA-69017
Reactome: R-HSA-8853884
Reactome: R-HSA-9687136
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Anaphase-Promoting Complex Subunit 10 antibody
Anti-Apc10 antibody
Anti-Cyclosome Subunit 10 antibody
Anti-ANAPC10 antibody
Anti-APC10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance