• Western blot analysis of lysates from wild type (WT) and ADK knockout (KO) HeLa cells, using [KO Validated] ADK Rabbit polyclonal antibody (STJ11100868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADK antibody (200-345) (STJ11100868)

SKU:
STJ11100868

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADK (200-345) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADK
Gene ID: 132
Uniprot ID: ADK_HUMAN
Immunogen Region: 200-345
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-345 of human ADK (NP_001114.2).
Immunogen Sequence: PFISQFYKESLMKVMPYVDI LFGNETEAATFAREQGFETK DIKEIAKKTQALPKMNSKRQ RIVIFTQGRDDTIMATESEV TAFAVLDQDQKEIIDTNGAG DAFVGGFLSQLVSDKPLTEC IRAGHYAASIIIRRTGCTFP EKPDFH
Tissue Specificity Widely expressed. Highest level in placenta, liver, muscle and kidney.
Function Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Protein Name Adenosine Kinase
Ak
Adenosine 5'-Phosphotransferase
Database Links Reactome: R-HSA-74217
Reactome: R-HSA-9755088
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Adenosine Kinase antibody
Anti-Ak antibody
Anti-Adenosine 5'-Phosphotransferase antibody
Anti-ADK antibody

Information sourced from Uniprot.org

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