| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:100-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ADAM17 |
| Gene ID: | 6868 |
| Uniprot ID: | ADA17_HUMAN |
| Immunogen Region: | 700-824 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 700-824 of human ADAM17 (NP_003174.3). |
| Immunogen Sequence: | KQYESLSLFHPSNVEMLSSM DSASVRIIKPFPAPQTPGRL QPAPVIPSAPAAPKLDHQRM DTIQEDPSTDSHMDEDGFEK DPFPNSSTAAKSFEDLTDHP VTRSEKAASFKLQRQNRVDS KETEC |
| Tissue Specificity | Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. Expressed in natural killer cells (at protein level). |
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at Thr-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding. |
| Function | Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity. Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R. Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. |
| Protein Name | Disintegrin And Metalloproteinase Domain-Containing Protein 17 Adam 17 Snake Venom-Like Protease Tnf-Alpha Convertase Tnf-Alpha-Converting Enzyme Cd Antigen Cd156b |
| Database Links | Reactome: R-HSA-1251985 Reactome: R-HSA-1442490 Reactome: R-HSA-177929 Reactome: R-HSA-193692 Reactome: R-HSA-2122948 Reactome: R-HSA-2644606 Reactome: R-HSA-2660826 Reactome: R-HSA-2691232 Reactome: R-HSA-2894862 Reactome: R-HSA-5362798 Reactome: R-HSA-75893 Reactome: R-HSA-9662834 Reactome: R-HSA-982772 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 17 antibody Anti-Adam 17 antibody Anti-Snake Venom-Like Protease antibody Anti-Tnf-Alpha Convertase antibody Anti-Tnf-Alpha-Converting Enzyme antibody Anti-Cd Antigen Cd156b antibody Anti-ADAM17 antibody Anti-CSVP antibody Anti-TACE antibody |
Information sourced from Uniprot.org